Coding

Part:BBa_K526001:Design

Designed by: Sung Kuk Lee   Group: iGEM11_UNIST_Korea   (2011-09-26)


ptrc*-Dpn


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1542
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 820


Design Notes

One should be careful while expressing Dpn genes. Dpn is supposed to chop up the DNA and cause cell death. Hence, no leaky level of Dpn is needed to recover live cells


Source

http://www.ncbi.nlm.nih.gov/nuccore/25307955?from=1637214&to=1637675

http://www.ncbi.nlm.nih.gov/nuccore/25307955?from=1637672&to=1638436

References